The aim of this research is to analyze the relations between personality traits and work alienation. This cross-sectional was carried out among nurses who work in teaching and research hospitals. A total of 600 (547 women/53 men) people participated in the study. The “Personal Information Form” was developed by the researcher, the “Work Alienation Scale” developed by Mottaz (1981), The “Five Factor Personality Features Scale” developed by John et al (1991), were also used. These are applied to the nurses which form the sample of the research. In the evaluation of the data; frequency analysis, correlation analysis, structural regression and path analysis were used. In the neurotic personality of personality traits of nurses had an influence on dimension of meaninglessness of work alienation and five other personality traits were found to have no significant effect on work alienation.