A positive teaching climate has an important impact on pupils’ attitude to learning subjects. The object of our research was to identify the factors affecting the climate of science subjects teaching. The research sample consisted of 454 primary and secondary school pupils. A questionnaire with 26 items was used as a research tool. Obtained data were subjected to factor analysis with loading factor of 0.45. Based on our research, four factors with a statistically significant impact on the climate of natural science subjects teaching was identified. At the same time, it was found that neither sex nor age of pupils has a statistically significant effect on the climate perception of natural science subjects teaching.