The development of medical technology has largely enhanced physicians’ professional responsibilities. Besides, the emergence of consumer rights, patients’ awakening of human right awareness, and the intervention of insurance have healthcare quality and physicians’ professional abilities gradually become the focus of health policies. The importance of continuing medical education is therefore highlighted. With nonequivalent pretest posttest control group design, two hospitals in Hubei Province are studied. One is proceeded continuing medical education, while the other maintains the original situation without continuing medical education. The experimental study is preceded for half a year. The research results show that 1.continuing medical education would affect job stress, 2.continuing medical education would influence sleep quality, 3.job stress presents significantly negative effects on sleeping time in sleep quality, and 4.job stress shows remarkably negative effects on quality level in sleep quality. According to the results, suggestions are proposed, expecting to enhance healthcare staff’s physiological and psychological health, promote job safety and performance, reduce medical incidents, and enhance healthcare service quality.