The purpose of this study is to explore the visual images of lotus ink-painting of visitors and to investigate the gender, age difference. The participant who attended 2016 Hsieh, Yi-E Lotus Ink-painting exhibition. The sample consisted 199 (male: 68, female: 131; age between 18~24 undergraduate student) valid questionnaires had been collected. Results indicate that: A Lotus Ink-painting of “Zen Sit “has the most Phlegmatic (t=-17.380, p=.000), Sorrowful (t=-9.999, p=.000) Meditation (t=-18.783, p=.000), Lonely (t=-10.803, p=.000) Plain (t=-18.747, p=.000) feeling. A Lotus Ink-painting of “Red Lotus” has the most Passionate (t=30.259, p=.000), Abundant (t=24.438, p=.000), Untrammeled (t=24.235, p=.000), Liberated (t=18.187, p=.000), Foison (t=21.306, p=.000) feeling. Gender and age difference do interaction significantly influence the visual images of lotus ink-painting. An artist can adopt the visual images of lotus ink-painting concluded form this study to understand the feelings of visitors on the lotus ink-painting.