The paper examines the empirically verified effect (parallel group experiment) of the application of the software form of integration and individualization of teaching of natural and mathematical knowledge in relation to traditional teaching, for example, the teaching sub-themes Cultivated habitats and life communities, and Equations with addition, subtraction, multiplication and division in the set N (the natural numbers) and N0 (the natural numbers plus the neutral element 0). The sample consisted of 250 pupils aged 10 to 11 years: 125 in the experimental group and 125 in the control group. The data were processed by statistical procedures and displayed in textual and graphical ways. The statistical analysis of the data obtained from the research has been achieved by the SPSS 17.0 software package, wherein the arithmetic mean, standard deviation, a t-test. Statistical indicators have confirmed the assumptions that the software form of integration and individualization contributes to more efficient and easier acquiring of knowledge, raising the quality and quantity of students’ knowledge and increasing the interest in studying content in the teaching of Knowledge of Nature and Mathematics, and as such points to a preferred model of teaching and learning.