This study aims to discuss the effects of E-Learning and cooperative learning on learning outcomes. E-Learning covers the dimensions of Interpersonal communication, abundant resources, Dynamic instruction, and Learning community; and, cooperative learning contains three dimensions of Cooperative motive, Social interaction, and Cognition construction. Teachers and students in ten public and private universities in southern Taiwan are selected as the research subjects. Total 500 questionnaires are distributed and collected on-site, and 327 valid copies are retrieved with the retrieval rate 65%. The research finding show partially significant correlations between E-Learning and cooperative learning, between cooperative learning and learning outcomes, and between E-Learning and learning outcomes. Finally, several suggestions are proposed for teachers in E-Learning and cooperative learning.